Cordovan Italian Queen April 2, 2012 • carl cook i have a hive that has nosema, i have treated it and they are now fine, but can the frames in it be used again next year in the spring time for splits. Meghan Hi Carl, yes, you can reuse the frames in splits or any other hive. Nosema is a common disease that is usually seasonal and if treated, as you have found, the bees will often recover. Only if your colony had a severe case and it died would we suggest you treat the frames. Rob Snyder Some beekeepers use phostoxin to fumigate comes to rid them of spores. I have also heard that using a bleach solution can help remove spores.