Oxalic Acid registered by EPA for use against Varroa mite on Honey bees

The varroa mite (Varroa destructor) has caused widespread devastation to honey bees through vampire-like feeding on larval and adult bees which decreases normal adult honey bee size, shortens their lifespan and can transfer viruses between bee hosts. (Rosenkrantz et al. 2010).

underside of varroa

Fig. 1 Underside of varroa mite (one leg is missing) taken using a scanning electronic microscope Photo credit: Bee Informed Partnership












Oxalic acid (CAS #144-62-7) has just been registered by the EPA for use on honey bee colonies here in the US. Oxalic acid has been legal to use on honeybees in Europe and Canada and is a naturally occurring chemical that can be found in a number of plants. It also occurs naturally in honey and is commonly used as wood bleach.

adult honey bee and varroa mite

Fig. 2. Adult female honey bee with a reddish brown oval shaped varroa mite on her thorax, between her wings, Photo credit: Bee Informed Partnership












A few oxalic acid applications have been registered:

“Application Methods and Labeling
Oxalic Acid will be labeled for application by three different methods:
1. By Solution to Package Bees (Oxalic acid in sugar solution is applied as a spray to the package)
2. By Solution to the Beehives (Oxalic acid in sugar solution is trickled between frames and other spaces)
3. Vapor treatment of Beehives (Oxalic acid dehydrate is heated and the vapor sublimates in the hive.”

**from J.E. Housenger, EPA, Registration Decision for the New Active Ingredient Oxalic Acid


The most useful application method for side-liner and hobby beekeepers is trickling the oxalic/sugar syrup mixture between the frames.  The product label includes important information on dose and personal protection equipment including, but not limited to: gloves, goggles and a respirator. It is particularly important to not breathe in the dust when you are preparing the solution. Be certain to read and follow the label directions to ensure the best results when treating your bees.

honey bee pupae with varroa mite

Fig. 3 Uncapped Honeybee pupa opened from the side with reddish brown adult varroa mite and bee-colored deutonymph (baby) mites Photo credit: Abdullah Ibrahim










Always be sure to monitor your bees for varroa mites so that you know the mite load before you prophylactically treat your colonies. If you treat with the same synthetic chemical at each treatment, this continual exposure may cause the mites to develop resistance to that specific varroacide.

Additionally, treatments can negatively impact honey bee larval development and adult life span and should be applied only after the mite levels have crossed the threshold for treatment. That threshold is different for different operations.  It is not likely that mites will develop resistance to a naturally occurring compound such as formic or oxalic acid as these acids desiccate mites and it is difficult to see a resistance developed to this mode of action.

Honey bee larvae with mites in celss

Fig. 4 White larval honey bees with a few cells infested with varroa mites Photo credit: Gary Reuter













***Check back here next Friday to learn how to non-destructively test for mites.

Rosenkrantz, P. , P. Aumeier, B. Ziegelmann 2010. Biology and control of Varroa destructor. Journal of
Invertebrate Pathology, 103: S96-S119.

Written By: Chris Kulhanek

Chris Kulhanek has written 3 post in this blog.

Chris has been keeping bees for 5 years and has worked for the University of Minnesota Bee Lab for the last three bee seasons. She assisted in fieldwork with Bee Informed Partnership for the last two years. She has a Bachelor of Science in Biology, with a Chemistry minor, and a Masters of Science in Entomology. She particularly enjoys sharing her adoration for insects with others and is proud she has been a "weird bug girl" for more than 20 years.